In Osaka during the Edo period, ruler Sanada Yukimura was in losing position, fearing Toyotomi's blood descendents would end, he impregnates one of five Shinano female ninja with the seed of Toyotomi Hideyoshi in hopes of continuing the bloodline. Princess Sen and the five female ninja escape from Osaka castle by blending in with the handmaidens. Lord Tokugawa Ieyasu who was supposed to rule all under the heavens was informed of this secret. So Ieyasu orders five samurai of the Iga Ninja clan (brought by Hattori Hanzo) to stop the Toyotomi bloodline by killing the Kunoichi ninja. An attack of ninja magic arts unfolds in a battle of the sexes.
Frustrated with the bad behavior of his lord, Inaba Yajuro (Otomo) declares that he is leaving the clan and sets off on a journey that leads him into a small town besieged by a violent group of brigands. In a kind of homage to Kurosawa's 7 Samurai, the townspeople have been unable to defend themselves and Inaba sets out to teach them how to stand up for themselves. Meanwhile the vile Lord Yasumasa has sent the 4 finest swordsmen in the clan out to hunt Inaba down and kill him. Led by the magnificent Ichibei (Konoe), a master of weaponry in his own right, everything points to an ultimate showdown between the former friends in a battle to the death.
The second film in the 20 Duels of Young Shingo trilogy directed by Sadatsugu Matsuda.