It is 300 years into the future. Earth's environment had been devastated by mankind's own foolish plans and humankind is beleaguered by the sentient forests which they have awoken. The world balance is tipped when a young boy named Agito stumbles across a machine that glowed in a strange blue hue inside a forbidden sanctuary.
Tezuka Osamu's most famous work "Jungle Emperor Leo" has been made into an animated version a number of times. In this theater version, Tezuka Osamu was able for the first time to depict the theme that "All life is equal" through Leo's self-sacrifice.
Two years after the events of Galaxy Express 999, Earth has become a battlefield, and Tetsuro is summoned to board the Three-Nine once more. In this, the shattering, full-length theatrical conclusion to Leiji Matsumoto's epic story, all questions will be answered and all mysteries will be revealed as Tetsuro embarks on a journey which will reveal a secret so awful, even Maetel herself can hardly bear to speak of it.